IL-1β (interleukin-1β) stimulates the production and release of multiple cytokines and chemokines by human preadipocytes

Alomar, Suliman Yousef and Gentili, Alessandra and Zaibi, Mohamed S. and Kępczyńska, Malgorzata A. and Trayhurn, Paul (2016) IL-1β (interleukin-1β) stimulates the production and release of multiple cytokines and chemokines by human preadipocytes. Archives of Physiology and Biochemistry. ISSN 1381-3455 (Print), 1744-4160 (Online)

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Abstract

The effect of IL-1β on cytokine and chemokine production by human preadipocytes has been examined. Preadipocytes were incubated with IL-1β and cytokine and chemokine release measured at 24 h by protein arrays, while the expression of cytokine/chemokine genes was assessed by qPCR at 4 and 24 h. IL-1β stimulated the secretion of multiple cytokines/chemokines, including IL-6, IL-8, IL-10, IL-13, MCP-4, TNFα and IP-10. IL-10 was not released by un-stimulated preadipocytes, while IL-6 exhibited the greatest response to IL-1β (453-fold increase). IL-16 and IL-12p40 did not respond to IL-1β. qPCR demonstrated that IL-1β markedly stimulated CCL3, CSF3 and CXCL10expression at 4 h (>900-fold mRNA increase). A time-course indicated that while CCL13 (encoding MCP-4) exhibited minimal basal expression in preadipocytes, expression increased progressively following differentiation. Human preadipocytes are highly sensitive to IL-1β, the cytokine stimulating a major inflammatory response in these cells similar to that in mature adipocytes.

Item Type: Article
Uncontrolled Keywords: Adipocytes; adipose tissue; gene expression; inflammation; protein arrays
Subjects: Q Science > QP Physiology
R Medicine > RB Pathology
Divisions: School of Science > Metabolic Research
Depositing User: Paul Trayhurn
Date Deposited: 14 Apr 2016 10:56
Last Modified: 03 Feb 2017 12:28
URI: http://bear.buckingham.ac.uk/id/eprint/113

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